rpe 65 (Novus Biologicals)

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Novus Biologicals rpe 65
Rpe 65, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rpe+65/RPE65+Antibody+(401%2E8B11%2E3D9)+-+BSA+Free/pm39384746-140-37-41
Average 95 stars, based on 45 article reviews

rpe 65 - by Bioz Stars, 2026-10

95/100 stars

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Incubation:

Article Title: Equivalence of conventionally-derived and parthenote-derived human embryonic stem cells.
Article Snippet: .. Following permeabilization with 0.1% Triton-X100/PBS for 40 min, the cells were rinsed with PBS and incubated overnight at 4uC in a humidified chamber with the primary antibody: ZO1 (1:50, Invitrogen), RPE-65 (1:250, Novus Biologicals), Bestrophin (1:25, Novus Biologicals), Occludin (1:50, Santa Cruz Biotechnology, Inc.) and CRALBP (1:50, Santa Cruz Biotechnology, Inc.). .. The cells were rinsed three times with PBS for 15 min and incubated at PLoS ONE | www.plosone.org 3 January 2011 | Volume 6 | Issue 1 | e14499 room temperature for 1 h with the secondary antibody: Alexa Fluor 546 donkey anti-mouse, Alexa Fluor 488 donkey antimouse, Alexa Fluor 546 donkey anti-rabbit or Alexa Fluor 488 donkey anti-goat (1:1000, Invitrogen).

Article Title: Equivalence of Conventionally-Derived and Parthenote-Derived Human Embryonic Stem Cells
Article Snippet: After electrophoresis, proteins were transferred to a Hybond-P polyvinylidene difluoride (PVDF) membrane (GE Healthcare Biosciences). .. Membranes were blocked for 2 hours in 5% nonfat milk in PBS-0.05% Tween-20 and incubated 2 hours in 5% nonfat milk containing primary antibodies: RPE-65 (1∶5000, Novus Biologicals), Bestrophin (1∶1000, Novus Biologicals) or NCAM1 (1∶2000, Santa Cruz Biotechnology, Inc.). .. Membranes were washed in PBS-0.05% Tween-20, then incubated for 1 hour at room temperature with secondary HRP-conjugated antibody goat anti-mouse (1∶1000, Millipore).

Article Title: Regulation of T-lymphocyte CCL3 and CCL4 production by retinal pigment epithelial cells.
Article Snippet: RESULTS.. Coculture of RPE cells with activated lymphocytes resulted in a reduction in CCL3 and CCL4 production by lymphocytes, primarily by soluble mediators.. Soluble CD54 was markedly increased on coculture of lymphocytes with RPE cells.

Article Title: Equivalence of conventionally-derived and parthenote-derived human embryonic stem cells.
Article Snippet: After electrophoresis, proteins were transferred to a Hybond-P polyvinylidene difluoride (PVDF) membrane (GE Healthcare Biosciences). .. Membranes were blocked for 2 hours in 5% nonfat milk in PBS-0.05% Tween-20 and incubated 2 hours in 5% nonfat milk containing primary antibodies: RPE-65 (1:5000, Novus Biologicals), Bestrophin (1:1000, Novus Biologicals) or NCAM1 (1:2000, Santa Cruz Biotechnology, Inc.). .. Membranes were washed in PBS-0.05% Tween-20, then incubated for 1 hour at room temperature with secondary HRP-conjugated antibody goat anti-mouse (1:1000, Millipore).

Article Title: Equivalence of Conventionally-Derived and Parthenote-Derived Human Embryonic Stem Cells
Article Snippet: .. Following permeabilization with 0.1% Triton-X100/PBS for 40 min, the cells were rinsed with PBS and incubated overnight at 4°C in a humidified chamber with the primary antibody: ZO1 (1∶50, Invitrogen), RPE-65 (1∶250, Novus Biologicals), Bestrophin (1∶25, Novus Biologicals), Occludin (1∶50, Santa Cruz Biotechnology, Inc.) and CRALBP (1∶50, Santa Cruz Biotechnology, Inc.). .. The cells were rinsed three times with PBS for 15 min and incubated at room temperature for 1 h with the secondary antibody: Alexa Fluor 546 donkey anti-mouse, Alexa Fluor 488 donkey anti-mouse, Alexa Fluor 546 donkey anti-rabbit or Alexa Fluor 488 donkey anti-goat (1∶1000, Invitrogen).

Saline:

Staining:

Article Title: Annexin A2 promotes proliferative vitreoretinopathy in response to a macrophage inflammatory signal in mice.
Article Snippet: .. In the Anxa2-/- eye, SMA is expressed in blood vessels; CD68-positive cells are rare. g, h Staining for SMA (red, rabbit anti-SMA, Cell Signaling 19245 T; 1:300 with donkey anti-rabbit Cy3, Jackson ImmunoResearch Laboratories 711-165-152; 1:300) and RPE-65 (green, mouse anti-RPE-65, Novus NB100-355; 1:150 with donkey anti-mouse Alexa 647, InvitrogenA31571; 1:100) inAnxa2+/+ (g) andAnxa2-/- (h) dispase-injected eyes. ..

other:

Article Title: Co-grafts of Human Embryonic Stem Cell Derived Retina Organoids and Retinal Pigment Epithelium for Retinal Reconstruction in Immunodeficient Retinal Degenerate Royal College of Surgeons Rats
Article Snippet: RPE 65 , Mouse , RPE , 1:100 (fl. Ab) , Novus , NB100-35 , AB_350269.

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